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Home Page > Conjugated Biologicals Biotin/Peroxidase/Gold/Magnetic > Biotin Conjugated Biological > Biotin rp24 HIV-1 IIIB (Euk)

Biotin rp24 HIV-1 IIIB (Euk)

Price : $415.00
Unit Of Measure 50ug
Item# 1003-B
Spec Sheet :

Product Specifications:

Item# 1003-B: Biotin p24 HIV-1 IIIB

 

Mass/Vial: 50µg                                                                                 

 

Vol./Vial: 50ul,50ug protein                                                           

 

Diluent: 50mM Dodium Phosphate, 10mM NaC1,pH7.5                      

 

Stabilizer: BSA                                                                                 

 

Preservative: 0.02%                                                                               

 

Storage: 4°C                                                                            

 

Stability: 12 months at 4°C

 

Minimum Dilution: 1 : 1000

 

Application: T-Cell Research, Diagnostics, Drug Screening, ELISA, Western ELISA

 

Description: Biotin conjugated to recombinant (baculovirus) HIV-1p24.

 

Coupling Ratio: Approximately 10-20 moles of biotin per mole p24. 

 

Specificity: Biotin p24 binds to anti-p24 monoclonal and polyclonal antibodies in Elisa and western Elisa as determined by strepavidin-AP/HRP assays.

 

Biological ActivityBiotin p24 binds to solid phase anti-p24 pAb (oligoclonal) as determined by capture ELISA using strepavidin-HRP as a secondary reagent.

 

Application and Instructions for use

 

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

1. ELISA may be performed in 1 : 5000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents.  p24/Anti-p24 pAb capture ELISA may be performed in 1-10ng pAb in solid phase and 0.1-1ng of biotin-p24.

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